A single-tube nested PCR for Pneumocystis carinii f. sp. hominis
نویسندگان
چکیده
منابع مشابه
Typing of Pneumocystis carinii f. sp. hominis by single-strand conformation polymorphism of four genomic regions.
To better investigate Pneumocystis carinii f. sp. hominis epidemiology, we have developed a molecular typing method. Because of the limited genetic variability of the P. carinii hominis genome, a multitarget approach was used. Four variable regions of the genome were amplified by PCR, polymorphism in each region was assessed by the single-strand conformation polymorphism (SSCP) technique, and t...
متن کاملDevelopment of a rapid real-time PCR assay for quantitation of Pneumocystis carinii f. sp. carinii.
A method for reliable quantification of Pneumocystis carinii in research models of P. carinii pneumonia (PCP) that is more convenient and reproducible than microscopic enumeration of organisms would greatly facilitate investigations of this organism. We developed a rapid quantitative touchdown (QTD) PCR assay for detecting P. carinii f. sp. carinii, the subspecies of P. carinii commonly used in...
متن کاملDevelopment and characterization of a molecular viability assay for Pneumocystis carinii f sp hominis.
Pneumocystis carinii pneumonia (PCP) remains the most common opportunistic infection among human immunodeficiency virus-infected persons. Despite this, little is known concerning the transmission dynamics of this infection. In the absence of a reliable method to isolate and culture P. carinii from environmental samples, it has not been possible to assess the importance of person-to-person trans...
متن کاملDetermination of copy number of rRNA genes in Pneumocystis carinii f. sp. hominis.
Differential PCR was performed to determine the copy number of rRNA genes in Pneumocystis carinii f. sp. hominis. Two different reference genes, thymidylate synthase (TS) and beta-tubulin (BTU) genes, were used. Primers for the internal transcribed spacer (ITS) region of nuclear rRNA genes and either the TS or BTU gene were mixed together to perform PCR on seven different bronchoalveolar lavage...
متن کاملDihydrofolate reductase gene polymorphisms in Pneumocystis carinii f. sp. hominis in Japan.
This study examined polymorphisms in the dihydrofolate reductase (DHFR) gene of Pneumocystis carinii isolates from 27 patients with P. carinii pneumonia (PCP) in Japan. Four substitution sites with two synonymous and two non-synonymous changes were found. Two synonymous substitutions at nucleotide positions 540 and 312 were identified in one and 13 patients, respectively. Two amino acid substit...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1997
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.35.6.1597-1599.1997